IHC-plus(tm) CXCL1 / GRO Alpha Antibody

LifeSpan BioSciences

IHC-plus(tm) CXCL1 / GRO Alpha Antibody

23

Immunohistochemistry: LS-B2843 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2843 was determined to be 5 ug/ml. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGRO/MGSA 100 ng/ml), a concentration of 1.5 - 3.0 ug/ml of this antibody is required. ELISA: To detect hGRO/MGSA by direct ELISA (using 100 ul/well antibody solution) a concentration of at least 0.5 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGRO/MGSA. Western Blot: To detect hGRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO/MGSA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.2 µg/ml), IHC, IHC-P (5 µg/ml), Neut (1.5 - 3 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B2843 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2843 was determined to be 5 ug/ml. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGRO/MGSA 100 ng/ml), a concentration of 1.5 - 3.0 ug/ml of this antibody is required. ELISA: To detect hGRO/MGSA by direct ELISA (using 100 ul/well antibody solution) a concentration of at least 0.5 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGRO/MGSA. Western Blot: To detect hGRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO/MGSA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking

Research Use Only

Polyclonal

Unconjugated

CXCL1

C-X-C motif chemokine ligand 1

FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1, growth-regulated alpha protein, C-X-C motif chemokine 1, GRO-alpha(1-73), GRO1 oncogene (melanoma growth stimulating activity, alpha), GRO1 oncogene (melanoma growth-stimulating activity), MGSA alpha, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha), fibroblast secretory protein, melanoma growth stimulating activity, alpha, melanoma growth stimulatory activity alpha, neutrophil-activating protein 3

Rabbit

Human

-20°C,2°C to 8°C

41116161