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IHC-plus(tm) CXCL4 / PF4 Antibody

LS-B14368
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPF4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) CXCL4 / PF4 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use as a source of sheep antibodies to human PF4.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (5 µg/ml) Suitable for use as a source of sheep antibodies to human PF4.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    10 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5196
  • Target name
    PF4
  • Target description
    platelet factor 4
  • Target synonyms
    CXCL4, PF-4, SCYB4, platelet factor 4, C-X-C motif chemokine 4, chemokine (C-X-C motif) ligand 4, iroplact, oncostatin-A
  • Host
    Sheep
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (PB10011). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 293T whole cell lysates, Lane 2: A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody (Catalog # PB10011) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PF4 at approximately 26 kDa. The expected band size for PF4 is at 11 kDa.
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IHC image of CSB-PA017809LA01HU diluted at 1:200 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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