IHC-plus(tm) CXCL7 / PPBP Antibody

LifeSpan BioSciences

IHC-plus(tm) CXCL7 / PPBP Antibody

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Immunohistochemistry: LS-B7394 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hNAP-2 (100 ng/ml), a concentration of 5-10 ug/ml of this antibody is required. ELISA: To detect hNAP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human NAP-2 (LS-C104604) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNAP-2. Western Blot: To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B7394 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hNAP-2 (100 ng/ml), a concentration of 5-10 ug/ml of this antibody is required. ELISA: To detect hNAP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human NAP-2 (LS-C104604) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNAP-2. Western Blot: To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

PPBP

pro-platelet basic protein

B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, CXCL7, LA-PF4, LDGF, MDGF, NAP-2, PBP, SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1, platelet basic protein, C-X-C motif chemokine 7, CXC chemokine ligand 7, beta-thromboglobulin, chemokine (C-X-C motif) ligand 7, connective tissue-activating peptide III, leukocyte-derived growth factor, low-affinity platelet factor IV, macrophage-derived growth factor, neutrophil-activating peptide 2, small inducible cytokine B7, small inducible cytokine subfamily B, member 7, thrombocidin 1, thrombocidin 2, thromboglobulin, beta-1

Rabbit

Human

41116161