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ProductsBack/IHC-plus(tm) DEPTOR / DEPDC6 Antibody

IHC-plus(tm) DEPTOR / DEPDC6 Antibody

LS-B12065
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDEPTOR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) DEPTOR / DEPDC6 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry at 5 ug/mL. Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 45 kDa Observed: 42 kDa. ELISA, IHC, IHC-P (10 µg/ml), WB (1 - 2 µg/ml) DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry at 5 ug/mL. Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 45 kDa Observed: 42 kDa
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64798
  • Target name
    DEPTOR
  • Target description
    DEP domain containing MTOR interacting protein
  • Target synonyms
    DEP.6, DEPDC6, hDEPTOR, DEP domain-containing mTOR-interacting protein, DEP domain containing 6, DEP domain-containing protein 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DEPDC6/DEPTOR using anti-DEPDC6/DEPTOR antibody (A03811-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat skeletal muscle tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DEPDC6/DEPTOR antigen affinity purified polyclonal antibody (A03811-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DEPDC6/DEPTOR at approximately 48 kDa. The expected band size for DEPDC6/DEPTOR is at 46 kDa.
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