IHC-plus(tm) DLD / Diaphorase / E3 Antibody
LS-B1443
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Porcine
TargetDLD
Overview
- SupplierLifeSpan BioSciences
- Product NameIHC-plus(tm) DLD / Diaphorase / E3 Antibody
- Delivery Days Customer23
- Application Supplier NoteImmunohistochemistry: LS-B1443 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1443 was determined to be 5 ug/ml.. ELISA, IF, IHC, IHC-P (5 µg/ml), IP, WB Immunohistochemistry: LS-B1443 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1443 was determined to be 5 ug/ml.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration10 mg/ml
- ConjugateUnconjugated
- Gene ID1738
- Target nameDLD
- Target descriptiondihydrolipoamide dehydrogenase
- Target synonymsDLDD, DLDH, E3, GCSL, LAD, OGDC-E3, PHE3, dihydrolipoyl dehydrogenase, mitochondrial, E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex, diaphorase, epididymis secretory sperm binding protein, glycine cleavage system L protein, glycine cleavage system protein L, lipoamide dehydrogenase, lipoamide reductase, lipoyl dehydrogenase, pyruvate dehydrogenase complex subunit E3, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex, glycine cleavage system protein L
- HostRabbit
- ReactivityHuman, Porcine
- Storage Instruction2°C to 8°C or -20°C
- UNSPSC41116161





![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody [N1N3] (GTX101232) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101232/GTX101232_40667_20181026_WB_R_w_23060100_154.webp)