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ProductsBack/IHC-plus(tm) EMA / MUC1 Antibody

IHC-plus(tm) EMA / MUC1 Antibody

LS-B10124
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetMUC1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) EMA / MUC1 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    MUC1 antibody can be used for detection of MUC1 by Western blot at 1 - 2 ug/ml. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Western Blot: Predicted: 53 (+ multiple others) kDa Observed:42, 120 kDa. ELISA, IHC, IHC-P (10 µg/ml), WB (1 - 2 µg/ml) MUC1 antibody can be used for detection of MUC1 by Western blot at 1 - 2 ug/ml. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Western Blot: Predicted: 53 (+ multiple others) kDa Observed:42, 120 kDa
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4582
  • Target name
    MUC1
  • Target description
    mucin 1, cell surface associated
  • Target synonyms
    ADMCKD, ADMCKD1, ADTKD2, CA 15-3, CD227, Ca15-3, EMA, H23AG, KL-6, MAM6, MCD, MCKD, MCKD1, MUC-1, MUC-1/SEC, MUC-1/X, MUC1/ZD, PEM, PEMT, PUM, mucin-1, H23 antigen, breast carcinoma-associated antigen DF3, cancer antigen 15-3, carcinoma-associated mucin, episialin, krebs von den Lungen-6, mucin 1, transmembrane, peanut-reactive urinary mucin, polymorphic epithelial mucin, tumor associated epithelial mucin, tumor-associated epithelial membrane antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of MUC1 using anti-MUC1 antibody (A00187-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC1 antigen affinity purified polyclonal antibody (Catalog # A00187-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC1 at approximately 122KD. The expected band size for MUC1 is at 122KD.
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