IHC-plus(tm) Endostatin Antibody
LS-B8215
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCOL18A1
Overview
- SupplierLifeSpan BioSciences
- Product NameIHC-plus(tm) Endostatin Antibody
- Delivery Days Customer23
- Application Supplier NoteImmunohistochemistry: LS-B8215 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. ELISA: To detect hEndostatin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Endostatin (LS-C104550) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hEndostatin. Western Blot: To detect hEndostatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEndostatin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B8215 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. ELISA: To detect hEndostatin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Endostatin (LS-C104550) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hEndostatin. Western Blot: To detect hEndostatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEndostatin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Estimated Purity...
- Gene ID80781
- Target nameCOL18A1
- Target descriptioncollagen type XVIII alpha 1 chain
- Target synonymsGLCC, KNO, KNO1, KS, collagen alpha-1(XVIII) chain, antiangiogenic agent, collagen, type XVIII, alpha 1, endostatin, multi-functional protein MFP
- HostRabbit
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C,RT
- UNSPSC12352203






