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ProductsBack/IHC-plus(tm) Eomesodermin / EOMES Antibody

IHC-plus(tm) Eomesodermin / EOMES Antibody

LS-B6801
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetEOMES
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) Eomesodermin / EOMES Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Western Blot and Immunohistochemistry. L. Western Blot: 0.5 ug/ml. Immunohistochemistry: 1:100. Does not work for IHC with mouse samples.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (10 µg/ml), WB (0.5 µg/ml) Suitable for use in Western Blot and Immunohistochemistry. L. Western Blot: 0.5 ug/ml. Immunohistochemistry: 1:100. Does not work for IHC with mouse samples.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8320
  • Target name
    EOMES
  • Target description
    eomesodermin
  • Target synonyms
    TBR2, eomesodermin homolog, T-box brain protein 2
  • Host
    Chicken
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TBR2 / Eomes antibody (GTX132776) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Figure 1. Western blot analysis of TBR2/Eomes using anti-TBR2/Eomes antibody (A00992-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBR2/Eomes antigen affinity purified polyclonal antibody (Catalog # A00992-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBR2/Eomes at approximately 85KD. The expected band size for TBR2/Eomes is at 73KD.
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