IHC-plus(tm) ERCC1 Antibody (aa172-184)
LS-B3956
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mammals, Monkey, Mouse, Rat
TargetERCC1
Overview
- SupplierLifeSpan BioSciences
- Product NameIHC-plus(tm) ERCC1 Antibody (aa172-184)
- Delivery Days Customer23
- Application Supplier NoteImmunohistochemistry: LS-B3956 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3956 was determined to be 3.75 ug/ml.. IF (10 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:4000) Immunohistochemistry: LS-B3956 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3956 was determined to be 3.75 ug/ml.
- ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.5 mg/ml
- ConjugateUnconjugated
- Gene ID2067
- Target nameERCC1
- Target descriptionERCC excision repair 1, endonuclease non-catalytic subunit
- Target synonymsCOFS4, RAD10, UV20, DNA excision repair protein ERCC-1, excision repair cross-complementation group 1, excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
- HostGoat
- ReactivityBovine, Canine, Human, Mammals, Monkey, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC41116161

![ERCC1 antibody [N1C3] detects ERCC1 protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERCC1 protein stained by ERCC1 antibody [N1C3] (GTX110562) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110562/GTX110562_40401_20150324_IFA_w_23060500_388.webp)




