IHC-plus(tm) EZH2 Antibody (Internal)

LifeSpan BioSciences

IHC-plus(tm) EZH2 Antibody (Internal)

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Immunohistochemistry: LS-B2528 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2528 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml. Bands at ~100kD and ~125kD.. ELISA (0.1 - 1 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.5 - 2 µg/ml) Immunohistochemistry: LS-B2528 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2528 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml. Bands at ~100kD and ~125kD.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.25 mg/ml

Unconjugated

EZH2

enhancer of zeste 2 polycomb repressive complex 2 subunit

ENX-1, ENX1, EZH2b, KMT6, KMT6A, WVS, WVS2, histone-lysine N-methyltransferase EZH2, enhancer of zeste homolog 2, lysine N-methyltransferase 6

Rabbit

IgG

Human

-20°C,2°C to 8°C

41116161