IHC-plus(tm) F2 / Prothrombin / Thrombin Antibody

LifeSpan BioSciences

IHC-plus(tm) F2 / Prothrombin / Thrombin Antibody

23

Immunohistochemistry: LS-B2559 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2559 was determined to be 5 ug/ml.. ELISA (5 - 10 µg/ml), IHC, IHC-P (5 µg/ml), WB (10 µg/ml) Immunohistochemistry: LS-B2559 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2559 was determined to be 5 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

1 mg/ml

Unconjugated

F2

coagulation factor II, thrombin

PT, RPRGL2, THPH1, prothrombin, prepro-coagulation factor II, prothrombin B-chain, thrombin factor II

Mouse

IgG2a

Human

-20°C,2°C to 8°C

41116161