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ProductsBack/IHC-plus(tm) FAM50A Antibody (clone 5F10)

IHC-plus(tm) FAM50A Antibody (clone 5F10)

LS-B4481
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetFAM50A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) FAM50A Antibody (clone 5F10)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot (Cell lysate) - positive control PC-12. Western Blot (Cell lysate) - positive control Hela NE. Sandwich ELISA (Recombinant protein).
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (5 µg/ml), WB Western Blot (Cell lysate) - positive control PC-12. Western Blot (Cell lysate) - positive control Hela NE. Sandwich ELISA (Recombinant protein).
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5F10
  • Conjugate
    Unconjugated
  • Gene ID9130
  • Target name
    FAM50A
  • Target description
    family with sequence similarity 50 member A
  • Target synonyms
    9F, DXS9928E, HXC-26, HXC26, MRXSA, XAP5, protein FAM50A, Armfield X-linked mental retardation syndrome, protein XAP-5
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FAM50A using anti-FAM50A antibody (M12622). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM50A antigen affinity purified monoclonal antibody (Catalog # M12622) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAM50A at approximately 40 kDa. The expected band size for FAM50A is at 40 kDa.
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