IHC-plus(tm) FANCD2 Antibody (clone 103)
LS-B195
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetFANCD2
Overview
- SupplierLifeSpan BioSciences
- Product NameIHC-plus(tm) FANCD2 Antibody (clone 103)
- Delivery Days Customer23
- Application Supplier NoteImmunohistochemistry: LS-B195 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B195 was determined to be 2.5 ug/ml.. ELISA, IHC, IHC-P (2.5 µg/ml), WB Immunohistochemistry: LS-B195 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B195 was determined to be 2.5 ug/ml.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID103
- ConjugateUnconjugated
- Gene ID2177
- Target nameFANCD2
- Target descriptionFA complementation group D2
- Target synonymsFA-D2, FA4, FACD, FAD, FAD2, FANCD, Fanconi anemia group D2 protein, Fanconi anemia complementation group D2
- HostMouse
- IsotypeIgG
- ReactivityHuman
- Storage Instruction2°C to 8°C or -20°C
- UNSPSC41116161





![Wild-type (WT) and FANCD2 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FANCD2 antibody [103] (GTX70299) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX70299/GTX70299_41330_20180413_WB_KO_watermark_w_23061221_975.webp)