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ProductsBack/IHC-plus(tm) FBN2 / Fibrillin 2 Antibody

IHC-plus(tm) FBN2 / Fibrillin 2 Antibody

LS-B6338
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human
TargetFBN2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) FBN2 / Fibrillin 2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Immunohistochemistry, Immunocytochemistry, ELISA, Western Blot and Immunoprecipitation. ELISA: 1:1000-1:2000. Western Blot: 1:200-1:500. Immunohistochemistry/Immunocytochemistry: 1:50-1:100. Immunoprecipitation: 1:100-1:200.. ELISA (1:1000 - 1:2000), ICC (1:50 - 1:100), IHC, IHC-P (1:100), IP (1:100 - 1:200), WB (1:200 - 1:500) Suitable for use in Immunohistochemistry, Immunocytochemistry, ELISA, Western Blot and Immunoprecipitation. ELISA: 1:1000-1:2000. Western Blot: 1:200-1:500. Immunohistochemistry/Immunocytochemistry: 1:50-1:100. Immunoprecipitation: 1:100-1:200.
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2201
  • Target name
    FBN2
  • Target description
    fibrillin 2
  • Target synonyms
    CCA, DA9, EOMD, fibrillin-2, fibrillin 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Bovine, Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Fibrillin 2/FBN2 using anti-Fibrillin 2/FBN2 antibody (A03408-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibrillin 2/FBN2 antigen affinity purified polyclonal antibody (Catalog # A03408-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibrillin 2/FBN2 at approximately 315,250 kDa. The expected band size for Fibrillin 2/FBN2 is at 315 kDa.
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