IHC-plus(tm) FGFR4 Antibody (clone 4H2B10B2)
LS-B3447
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetFGFR4
Overview
- SupplierLifeSpan BioSciences
- Product NameIHC-plus(tm) FGFR4 Antibody (clone 4H2B10B2)
- Delivery Days Customer23
- Application Supplier NoteImmunohistochemistry: LS-B3447 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3447 was determined to be 1:200.. ELISA (1:10000), IHC, IHC-Fr, IHC-P (1:200), WB (1:100 - 1:500) Immunohistochemistry: LS-B3447 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3447 was determined to be 1:200.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4H2B10B2
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2264
- Target nameFGFR4
- Target descriptionfibroblast growth factor receptor 4
- Target synonymsCD334, JTK2, TKF, fibroblast growth factor receptor 4, hydroxyaryl-protein kinase, protein-tyrosine kinase, tyrosine kinase related to fibroblast growth factor receptor, tyrosylprotein kinase
- HostMouse
- IsotypeIgG1
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC41116161
![HepG2 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FGFR4 antibody [N3C2], Internal (GTX112935) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112935/GTX112935_40086_20170825_WB_Fraction_w_23060500_553.webp)



