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ProductsBack/IHC-plus(tm) FTL / Ferritin Light Chain Antibody

IHC-plus(tm) FTL / Ferritin Light Chain Antibody

LS-B11442
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetFTL
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) FTL / Ferritin Light Chain Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Recommended conditions for Immunohistochemistry: For FFPE tissues, perform steam-based antigen retrieval using Tris/EDTA buffer at pH 9.0.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (20 µg/ml), WB Recommended conditions for Immunohistochemistry: For FFPE tissues, perform steam-based antigen retrieval using Tris/EDTA buffer at pH 9.0.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2512
  • Target name
    FTL
  • Target description
    ferritin light chain
  • Target synonyms
    FTL1, LFTD, NBIA3, ferritin light chain, epididymis secretory sperm binding protein, ferritin L subunit, ferritin L-chain, ferritin light polypeptide-like 3, ferritin, light polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Ferritin Light Chain using anti-Ferritin Light Chain antibody (M01956-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ferritin Light Chain antigen affinity purified monoclonal antibody (Catalog # M01956-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ferritin Light Chain at approximately 20 kDa. The expected band size for Ferritin Light Chain is at 20 kDa.
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