IHC-plus(tm) GAPDH Antibody (clone 1D4)

LifeSpan BioSciences

IHC-plus(tm) GAPDH Antibody (clone 1D4)

14

GAPDH (1D4).

Immunohistochemistry: LS-B83 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B83 was determined to be 1.25-2.5 ug/ml.. IF (1:100), IHC, IHC-P (1.25 - 2.5 µg/ml), IP, WB (1:1000) Immunohistochemistry: LS-B83 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B83 was determined to be 1.25-2.5 ug/ml.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

1D4

1 mg/ml

Unconjugated

...

GAPDH

glyceraldehyde-3-phosphate dehydrogenase

aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; G3PD; GAPD; glyceraldehyde-3-phosphate dehydrogenase; HEL-S-162eP; OCAS, p38 component; Oct1 coactivator in S phase, 38 Kd component; peptidyl-cysteine S-nitrosylase GAPDH

Mouse

IgM

Avian, Bovine, Chicken, Hamster, Human, Mouse, Opossum, Porcine, Primate, Rabbit, Rat, Sheep

-20°C,2°C to 8°C

12352203