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IHC-plus(tm) GFI1 Antibody (aa280-330)

LS-B7250
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Chicken, Human, Mouse, Rat
TargetGFI1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) GFI1 Antibody (aa280-330)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (5 - 10 µg/ml), WB (6 - 8 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2672
  • Target name
    GFI1
  • Target description
    growth factor independent 1 transcriptional repressor
  • Target synonyms
    GFI-1, GFI1A, SCN2, ZNF163, zinc finger protein Gfi-1, growth factor independence-1, growth factor independent 1 transcription repressor, growth factor independent protein 1, zinc finger protein 163
  • Host
    Rabbit
  • Reactivity
    Bovine, Chicken, Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. IHC analysis of GFI1 using anti-GFI1 antibody (A00888-1). GFI1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-GFI1 Antibody (A00888-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GFI1 antibody (GTX133798) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Lane 1: Mouse Lung lysates; Lane 2: Mouse Lymph node lysates probed with GFI1/ZNF163 Polyclonal Antibody, Unconjugated (bs-13337R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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