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ProductsBack/IHC-plus(tm) GSTA3 Antibody (clone 1F11)

IHC-plus(tm) GSTA3 Antibody (clone 1F11)

LS-B4337
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetGSTA3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) GSTA3 Antibody (clone 1F11)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot (Cell lysate) - positive control HepG2.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (5 µg/ml), WB Western Blot (Cell lysate) - positive control HepG2.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1F11
  • Conjugate
    Unconjugated
  • Gene ID2940
  • Target name
    GSTA3
  • Target description
    glutathione S-transferase alpha 3
  • Target synonyms
    GSTA3-3, GTA3, glutathione S-transferase A3, GST class-alpha member 3, S-(hydroxyalkyl)glutathione lyase A3, glutathione S-alkyltransferase A3, glutathione S-aralkyltransferase A3, glutathione S-aryltransferase A3, glutathione S-transferase A3-3
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Gsta3 using anti-Gsta3 antibody (A05829-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat ovary tissue lysates, Lane 3: rat brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gsta3 antigen affinity purified polyclonal antibody (Catalog # A05829-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gsta3 at approximately 28KD. The expected band size for Gsta3 is at 28KD.
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