IHC-plus(tm) HIF3A / HIF3-Alpha Antibody

LifeSpan BioSciences

IHC-plus(tm) HIF3A / HIF3-Alpha Antibody

23

Immunohistochemistry: LS-B714 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B714 was determined to be 5 ug/ml.***Does not work to detect human protein in Western blot.***. ELISA (1:14000 - 1:80000), IHC, IHC-P (5 µg/ml), WB (1:1000 - 1:8000) Immunohistochemistry: LS-B714 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B714 was determined to be 5 ug/ml.***Does not work to detect human protein in Western blot.***

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1.2 mg/ml

Unconjugated

HIF3A

hypoxia inducible factor 3 subunit alpha

HIF-3A, HIF3-alpha-1, IPAS, MOP7, PASD7, bHLHe17, hypoxia-inducible factor 3-alpha, PAS domain-containing protein 7, basic-helix-loop-helix-PAS protein MOP7, class E basic helix-loop-helix protein 17, hypoxia inducible factor 3 alpha subunit, inhibitory PAS domain protein, member of PAS protein 7

Rabbit

IgG

Human, Mouse

-20°C,2°C to 8°C

41116161