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ProductsBack/IHC-plus(tm) HIP1 Antibody (clone 1F12)

IHC-plus(tm) HIP1 Antibody (clone 1F12)

LS-B6765
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetHIP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) HIP1 Antibody (clone 1F12)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (10 µg/ml), IP, WB Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1F12
  • Conjugate
    Unconjugated
  • Gene ID3092
  • Target name
    HIP1
  • Target description
    huntingtin interacting protein 1
  • Target synonyms
    HIP-I, ILWEQ, SHON, SHONbeta, SHONgamma, huntingtin-interacting protein 1, huntingtin-interacting protein I
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of HIP1 using anti-HIP1 antibody (M02242). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIP1 antigen affinity purified monoclonal antibody (Catalog # M02242) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIP1 at approximately 116 kDa. The expected band size for HIP1 is at 116 kDa.
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