IHC-plus(tm) HPSE / Heparanase Antibody

LifeSpan BioSciences

IHC-plus(tm) HPSE / Heparanase Antibody

23

Immunohistochemistry: LS-B2441 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2441 was determined to be 5 ug/ml. Western Blot: 1:1000.. IHC, IHC-P (5 µg/ml), WB (1:2000) Immunohistochemistry: LS-B2441 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2441 was determined to be 5 ug/ml. Western Blot: 1:1000.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

5.7 mg/ml

Unconjugated

HPSE

heparanase

HPA, HPA1, HPR1, HPSE1, HSE1, heparanase, endo-glucoronidase, heparanase-1

Rabbit

IgG

Human, Mouse

-20°C,2°C to 8°C

41116161