More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/IHC-plus(tm) HSF1 Antibody (aa270-319)

IHC-plus(tm) HSF1 Antibody (aa270-319)

LS-B7569
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetHSF1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) HSF1 Antibody (aa270-319)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot: Predicted MW 57 kDa
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100), Peptide-ELISA (1:10000), WB (1:500 - 1:1000) Western Blot: Predicted MW 57 kDa
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3297
  • Target name
    HSF1
  • Target description
    heat shock transcription factor 1
  • Target synonyms
    HSTF1, heat shock factor protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
HSF1 AntibodyCAB13765
ReactivityHuman
TargetHSF1
  • SizePrice
Western Blot analysis of COLO 293T cells using HSF1 Polyclonal Antibody
Product group Antibodies
Cusabio
HSF1 AntibodyCSB-PA002974
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetHSF1
  • SizePrice
Product group Antibodies
Biomatik
HSF1 Polyclonal AntibodyCAC14986
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetHSF1
  • SizePrice
Figure 1. Western blot analysis of HSF1 using anti-HSF1 antibody (A00250). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF1 antigen affinity purified polyclonal antibody (Catalog # A00250) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF1 at approximately 80 kDa. The expected band size for HSF1 is at 57 kDa.
Product group Antibodies
Boster Bio
Anti-HSF1 Antibody Picoband(r)A00250-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetHSF1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-HSF1 Antibody144-60474
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHSF1
  • SizePrice
Product group Antibodies
Abbexa
HSF1 Sumoylation Site AntibodyABX031603
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • SizePrice
Anti-HSF1 Antibody
Product group Antibodies
Antibodies.com
Anti-HSF1 AntibodyA98093
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
  • SizePrice
Product group Antibodies
MedChem Express
Phospho-HSF1 (Ser303/Ser307) Antibody (YA188, Rabbit)HY-P80822
TargetHSF1
  • SizePrice
Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.
Product group Antibodies
Atlas Antibodies
Anti-HSF1 AntibodyHPA008888
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetHSF1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
HSF1 AntibodyLS-C400798
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetHSF1
  • SizePrice