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ProductsBack/IHC-plus(tm) HSF1 Antibody (clone 10H8)

IHC-plus(tm) HSF1 Antibody (clone 10H8)

LS-B8141
LifeSpan BioSciences
ApplicationsGel Shift Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Guinea Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat
TargetHSF1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) HSF1 Antibody (clone 10H8)
  • Delivery Days Customer
    23
  • Applications
    Gel Shift Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, GS, ICC, IF, IHC, IHC-P (10 µg/ml), IP, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    10H8
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3297
  • Target name
    HSF1
  • Target description
    heat shock transcription factor 1
  • Target synonyms
    HSTF1, heat shock factor protein 1
  • Host
    Rat
  • Isotype
    IgG1
  • Reactivity
    Bovine, Guinea Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of HSF1 using anti-HSF1 antibody (A00250). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF1 antigen affinity purified polyclonal antibody (Catalog # A00250) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF1 at approximately 80 kDa. The expected band size for HSF1 is at 57 kDa.
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