IHC-plus(tm) HSF1 Antibody

LifeSpan BioSciences

IHC-plus(tm) HSF1 Antibody

23

Immunohistochemistry: LS-B2682 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2682 was determined to be 5 ug/ml. Western Blot: Positive control: Recombinant Human HSF-1 Protein.. IHC, IHC-P (5 µg/ml), WB (1:500) Immunohistochemistry: LS-B2682 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2682 was determined to be 5 ug/ml. Western Blot: Positive control: Recombinant Human HSF-1 Protein.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

...

HSF1

heat shock transcription factor 1

HSTF1, heat shock factor protein 1

Rabbit

IgG

Human, Monkey, Mouse, Rabbit, Rat

2°C to 8°C or -20°C

12352203