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ProductsBack/IHC-plus(tm) IL-33 Antibody

IHC-plus(tm) IL-33 Antibody

LS-B15774
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetIL33
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) IL-33 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 16kDa/25kDa/26kDa/30kDa, while the observed MW by Western blot was 35kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:200), WB (1:200 - 1:2000) The predicted MW is 16kDa/25kDa/26kDa/30kDa, while the observed MW by Western blot was 35kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID90865
  • Target name
    IL33
  • Target description
    interleukin 33
  • Target synonyms
    C9orf26, DVS27, IL1F11, NF-HEV, NFEHEV, interleukin-33, DVS27-related protein, interleukin-1 family member 11, nuclear factor for high endothelial venules, nuclear factor from high endothelial venules
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of IL33 using anti-IL33 antibody (A00113). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IL33 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL33 antigen affinity purified polyclonal antibody (Catalog # A00113) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL33 at approximately 18KD. The expected band size for IL33 is at 18KD.
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