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ProductsBack/IHC-plus(tm) IL13 Antibody (clone 5J56)

IHC-plus(tm) IL13 Antibody (clone 5J56)

LS-B3767
LifeSpan BioSciences
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityRat
TargetIL13
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) IL13 Antibody (clone 5J56)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Flow Cytometry: 0.1-1ug per 10e6 cells for detection in PMA/ionophore-stimulated rat splenocytes. Dot Blot: 1:500-1:1000
  • Applications
    Flow Cytometry, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, Flo, IHC-P (10 µg/ml) Flow Cytometry: 0.1-1ug per 10e6 cells for detection in PMA/ionophore-stimulated rat splenocytes. Dot Blot: 1:500-1:1000
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5J56
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3596
  • Target name
    IL13
  • Target description
    interleukin 13
  • Target synonyms
    IL-13, P600, interleukin-13
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of IL13 using anti-IL13 antibody (A00077). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL13 antigen affinity purified polyclonal antibody (Catalog # A00077) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL13 at approximately 20KD. The expected band size for IL13 is at 16KD.
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