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ProductsBack/IHC-plus(tm) IL7R / CD127 Antibody

IHC-plus(tm) IL7R / CD127 Antibody

LS-B14308
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetIL7R
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) IL7R / CD127 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 28kDa/29kDa/34kDa/51kDa, while the observed MW by Western blot was 52kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:20 - 1:50), IHC (1:50 - 1:200), IHC-P (1:100), WB (1:500 - 1:2000) The predicted MW is 28kDa/29kDa/34kDa/51kDa, while the observed MW by Western blot was 52kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.72 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3575
  • Target name
    IL7R
  • Target description
    interleukin 7 receptor
  • Target synonyms
    CD127, CDW127, IL-7R-alpha, IL-7Ralpha, IL7RA, IL7Ralpha, ILRA, IMD104, lnc-IL7R, sIL-7R, interleukin-7 receptor subunit alpha, CD127 antigen, IL-7 receptor subunit alpha, IL-7R subunit alpha, interleukin 7 receptor alpha chain, soluble interleukin-7 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of CD127/IL7R using anti-CD127/IL7R antibody (A02222-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD127/IL7R antigen affinity purified polyclonal antibody (Catalog # A02222-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD127/IL7R at approximately 170 kDa. The expected band size for CD127/IL7R is at 52 kDa.
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