IHC-plus(tm) ING3 Antibody (aa285-310)
LS-B385
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetING3
Overview
- SupplierLifeSpan BioSciences
- Product NameIHC-plus(tm) ING3 Antibody (aa285-310)
- Delivery Days Customer23
- Application Supplier NoteImmunohistochemistry: LS-B385 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B385 was determined to be 2.5 ug/ml.. ELISA (1:10000 - 1:40000), IHC, IHC-P (2.5 µg/ml), WB (1:200 - 1:2000) Immunohistochemistry: LS-B385 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B385 was determined to be 2.5 ug/ml.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID54556
- Target nameING3
- Target descriptioninhibitor of growth family member 3
- Target synonymsEaf4, ING2, MEAF4, p47ING3, inhibitor of growth protein 3
- HostGoat
- IsotypeIgG
- ReactivityHuman
- Storage Instruction2°C to 8°C or -20°C
- UNSPSC41116161
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ING3 antibody [N2N3] (GTX102480) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102480/GTX102480_39617_20220908_WB_22091323_806.webp)



