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ProductsBack/IHC-plus(tm) MAP3K7 / TAK1 Antibody

IHC-plus(tm) MAP3K7 / TAK1 Antibody

LS-B18
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAP3K7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) MAP3K7 / TAK1 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    TAK1 antibody can be used for the detection of TAK1 by Western blot at 1 to 4 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Western Blot:. ELISA, IF (20 µg/ml), IHC, IHC-P (2.5 µg/ml), WB (0.5 - 1 µg/ml) TAK1 antibody can be used for the detection of TAK1 by Western blot at 1 to 4 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Western Blot:
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6885
  • Target name
    MAP3K7
  • Target description
    mitogen-activated protein kinase kinase kinase 7
  • Target synonyms
    CSCF, FMD2, MEKK7, TAK1, TGF1a, mitogen-activated protein kinase kinase kinase 7, TGF-beta activated kinase 1, transforming growth factor-beta-activated kinase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TAK1 using anti-TAK1 antibody (PB10006). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human A374 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAK1 antigen affinity purified polyclonal antibody (Catalog # PB10006) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAK1 at approximately 72 kDa. The expected band size for TAK1 is at 67 kDa.
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