IHC-plus(tm) MAPK6 / ERK3 Antibody (aa683-694)

LifeSpan BioSciences

IHC-plus(tm) MAPK6 / ERK3 Antibody (aa683-694)

23

Human MAPK6 / ERK3. Reported variants represent identical protein (NP_056621.4; NP_081694.1).

Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 ug/ml. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 ug EB11926. Western blots of the IP were labeled with rabbit anti-ERK3 serum (CST) or with mouse anti-MK5 (SC). An approx 100kD of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.. IHC, IHC-P (10 µg/ml), IP (1.5 µg/ml), Peptide-ELISA (1:128000), WB (0.5 - 1 µg/ml) Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 ug/ml. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 ug EB11926. Western blots of the IP were labeled with rabbit anti-ERK3 serum (CST) or with mouse anti-MK5 (SC). An approx 100kD of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.5 mg/ml

Unconjugated

MAPK6

mitogen-activated protein kinase 6

ERK3; ERK-3; extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p97; HsT17250; MAP kinase 6; MAPK 6; mitogen-activated protein kinase 6; p97MAPK; p97-MAPK; PRKM6; protein kinase, mitogen-activated 5; protein kinase, mitogen-activated 6

Goat

Bovine, Canine, Chicken, Equine, Hamster, Human, Mouse, Porcine, Rabbit

-20°C

12352203