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ProductsBack/IHC-plus(tm) MAT2A Antibody (Internal)

IHC-plus(tm) MAT2A Antibody (Internal)

LS-B10931
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Primate, Rat, Zebra Fish
TargetMAT2A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) MAT2A Antibody (Internal)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (5 µg/ml), WB (2 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4144
  • Target name
    MAT2A
  • Target description
    methionine adenosyltransferase 2A
  • Target synonyms
    MATA2, MATII, SAMS2, S-adenosylmethionine synthase isoform type-2, MAT 2, MAT-II, adoMet synthase 2, adoMet synthetase 2, methionine adenosyltransferase 2, methionine adenosyltransferase II, alpha, testicular tissue protein Li 121
  • Host
    Rabbit
  • Reactivity
    Bovine, Human, Mouse, Primate, Rat, Zebra Fish
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MAT2A using anti-MAT2A antibody (A04557-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAT2A antigen affinity purified polyclonal antibody (Catalog # A04557-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAT2A at approximately 50 kDa. The expected band size for MAT2A is at 44 kDa.
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