IHC-plus(tm) MCAM / CD146 Antibody (Tyr641)

LifeSpan BioSciences

IHC-plus(tm) MCAM / CD146 Antibody (Tyr641)

23

Immunohistochemistry: LS-B3628 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3628 was determined to be 10 ug/ml.. IHC, IHC-P (10 µg/ml), WB (1:1000) Immunohistochemistry: LS-B3628 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3628 was determined to be 10 ug/ml.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.25 mg/ml

Unconjugated

MCAM

melanoma cell adhesion molecule

CD146, HEMCAM, METCAM, MUC18, MelCAM, cell surface glycoprotein MUC18, Gicerin, S-endo 1 endothelial-associated antigen, cell surface glycoprotein P1H12, melanoma adhesion molecule, melanoma-associated antigen A32, melanoma-associated antigen MUC18

Rabbit

Human

-20°C,2°C to 8°C

41116161