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ProductsBack/IHC-plus(tm) MPO / Myeloperoxidase Antibody

IHC-plus(tm) MPO / Myeloperoxidase Antibody

LS-B6695
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetMPO
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) MPO / Myeloperoxidase Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    IHC: This antibody performs superbly in paraffin-embedded tissue sections fixed in formalin, frozen sections and cell cytospins. Antigen retrieval is essential for use on paraffin sections.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF, IHC, IHC-Fr, IHC-P (2.5 µg/ml), WB (10 - 50 µg/ml) IHC: This antibody performs superbly in paraffin-embedded tissue sections fixed in formalin, frozen sections and cell cytospins. Antigen retrieval is essential for use on paraffin sections.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4353
  • Target name
    MPO
  • Target description
    myeloperoxidase
  • Target synonyms
    myeloperoxidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of MPO using anti-MPO antibody (A00372-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPO antigen affinity purified polyclonal antibody (Catalog # A00372-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPO at approximately 55,80-90 kDa. The expected band size for MPO is at 84 kDa.
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HL-60 cells were fixed with 4% PFA for 10min at room temperature,20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MPO Polyclonal Antibody (bs-4943R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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