IHC-plus(tm) MTHFD1 Antibody (aa137-150)

LifeSpan BioSciences

IHC-plus(tm) MTHFD1 Antibody (aa137-150)

23

Immunohistochemistry: LS-B4110 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4110 was determined to be 3.75 ug/ml. Peptide ELISA: antibody detection limit dilution 1:1000. Western Blot: Approx. 110kD band observed in Human Brain (substantia nigra) lysates (calculated MW of 102kD according to NP_005947.2). Recommended concentration: 1-3 ug/ml.. IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:1000), WB (1 - 3 µg/ml) Immunohistochemistry: LS-B4110 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4110 was determined to be 3.75 ug/ml. Peptide ELISA: antibody detection limit dilution 1:1000. Western Blot: Approx. 110kD band observed in Human Brain (substantia nigra) lysates (calculated MW of 102kD according to NP_005947.2). Recommended concentration: 1-3 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.5 mg/ml

Unconjugated

MTHFD1

methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1

CIMAH, MTHFC, MTHFD, C-1-tetrahydrofolate synthase, cytoplasmic, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase, C1-THF synthase, cytoplasmic C-1-tetrahydrofolate synthase, epididymis secretory sperm binding protein, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase

Goat

Equine, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Rabbit

-20°C

41116161