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ProductsBack/IHC-plus(tm) NDUFA4 Antibody (aa32-81)

IHC-plus(tm) NDUFA4 Antibody (aa32-81)

LS-B6885
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCOXFA4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) NDUFA4 Antibody (aa32-81)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot: Predicted MW 9 kDa
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:200), Peptide-ELISA (1:10000), WB (1:500 - 1:1000) Western Blot: Predicted MW 9 kDa
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4697
  • Target name
    COXFA4
  • Target description
    cytochrome c oxidase associated subunit FA4
  • Target synonyms
    CI-9k, CI-MLRQ, MC4DN21, MISTR1, MLRQ, MRCAF1, NDUFA4, cytochrome c oxidase subunit NDUFA4, Complex I 9kDa subunit, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4, 9kDa, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4, NADH-ubiquinone oxidoreductase MLRQ subunit, NDUFA4 mitochondrial complex associated, complex I-MLRQ, cytochrome c oxidase subunit FA4, mitochondrial respiratory chain associated factor 1, mitochondrial stress response 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of NDUFA4 using anti-NDUFA4 antibody (A09276-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T98G whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA4 antigen affinity purified polyclonal antibody (Catalog # A09276-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA4 at approximately 12 kDa. The expected band size for NDUFA4 is at 9 kDa.
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