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ProductsBack/IHC-plus(tm) NEDD4 Antibody (aa725-775)

IHC-plus(tm) NEDD4 Antibody (aa725-775)

LS-B15099
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNEDD4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) NEDD4 Antibody (aa725-775)
  • Delivery Days Customer
    23
  • Applications
    ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (5 µg/ml), IP (2 - 10 µg/mg lysate) WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4734
  • Target name
    NEDD4
  • Target description
    NEDD4 E3 ubiquitin protein ligase
  • Target synonyms
    NEDD4-1, RPF1, E3 ubiquitin-protein ligase NEDD4, HECT-type E3 ubiquitin transferase NEDD4, cell proliferation-inducing gene 53 protein, neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase, receptor-potentiating factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NEDD4 using anti-NEDD4 antibody (A00984-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEDD4 antigen affinity purified polyclonal antibody (Catalog # A00984-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEDD4 at approximately 149KD. The expected band size for NEDD4 is at 149KD.
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