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ProductsBack/IHC-plus(tm) NFATC4 / NFAT3 Antibody (aa136-185)

IHC-plus(tm) NFATC4 / NFAT3 Antibody (aa136-185)

LS-B11342
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetNFATC4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) NFATC4 / NFAT3 Antibody (aa136-185)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot: Predicted MW 95 kDa
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:50), Peptide-ELISA (1:1000), WB (1:500 - 1:1000) Western Blot: Predicted MW 95 kDa
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4776
  • Target name
    NFATC4
  • Target description
    nuclear factor of activated T cells 4
  • Target synonyms
    NF-AT3, NF-ATC4, NFAT3, nuclear factor of activated T-cells, cytoplasmic 4, T-cell transcription factor NFAT3, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of NFATC4 using anti-NFATC4 antibody (A02599-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: human Hela whole cell lysates, Lane 4: human placenta tissue lysates, Lane 5: human A549 whole cell lysates, Lane 6: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFATC4 antigen affinity purified polyclonal antibody (Catalog # A02599-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFATC4 at approximately 95120KD. The expected band size for NFATC4 is at 95KD.
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