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ProductsBack/IHC-plus(tm) NNMT Antibody (clone 3D8)

IHC-plus(tm) NNMT Antibody (clone 3D8)

LS-B9960
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetNNMT
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) NNMT Antibody (clone 3D8)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:100), IF (1:100), IHC, IHC-P (20 µg/ml), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3D8
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4837
  • Target name
    NNMT
  • Target description
    nicotinamide N-methyltransferase
  • Target synonyms
    nicotinamide N-methyltransferase
  • Host
    Mouse
  • Isotype
    IgG2b
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NNMT using anti-NNMT antibody (A02676-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NNMT antigen affinity purified polyclonal antibody (Catalog # A02676-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NNMT at approximately 28 kDa. The expected band size for NNMT is at 30 kDa.
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