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ProductsBack/IHC-plus(tm) NPHS1 / Nephrin Antibody

IHC-plus(tm) NPHS1 / Nephrin Antibody

LS-B12155
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNPHS1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) NPHS1 / Nephrin Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Nephrin antibody can be used for detection of Nephrin by Western blot at 1 - 2 ug/ml. Antibody can also be used for immunohistochemistry at 5 ug/ml. For immunofluorescence start at 20 ug/mL Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 125, 137 kDa Observed: 125 kDa. ELISA, IF, IHC, IHC-P (10 µg/ml), WB (1 - 2 µg/ml) Nephrin antibody can be used for detection of Nephrin by Western blot at 1 - 2 ug/ml. Antibody can also be used for immunohistochemistry at 5 ug/ml. For immunofluorescence start at 20 ug/mL Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 125, 137 kDa Observed: 125 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4868
  • Target name
    NPHS1
  • Target description
    NPHS1 adhesion molecule, nephrin
  • Target synonyms
    CNF, NPHN, nephrin, nephrin, NPHS1, nephrin, nephrosis 1, congenital, Finnish type (nephrin), renal glomerulus-specific cell adhesion receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Nephrin using anti-Nephrin antibody (A01991). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nephrin antigen affinity purified polyclonal antibody (Catalog # A01991) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nephrin at approximately 185 kDa. The expected band size for Nephrin is at 135 kDa.
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