IHC-plus(tm) NUMB Antibody (aa600-653)

LifeSpan BioSciences

IHC-plus(tm) NUMB Antibody (aa600-653)

23

Immunohistochemistry: LS-B265 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B265 was determined to be 2.5 ug/ml.. IHC, IHC-P (2.5 µg/ml), WB (0.2 - 0.5 µg/ml) Immunohistochemistry: LS-B265 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B265 was determined to be 2.5 ug/ml.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

3.5 mg/ml

Unconjugated

NUMB

NUMB endocytic adaptor protein

C14orf41, S171, c14_5527, protein numb homolog, h-Numb, numb homolog

Rabbit

Human, Mouse

-80°C,2°C to 8°C

41116161