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ProductsBack/IHC-plus(tm) NUMB Antibody (aa638-651)

IHC-plus(tm) NUMB Antibody (aa638-651)

LS-B16964
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Rabbit, Xenopus
TargetNUMB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) NUMB Antibody (aa638-651)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 80 kDa band observed in Mouse Brain lysates (calculated MW of 70.8 kDa according to NP_001005743.1). Recommended concentration: 0.03-0.1 ug/ml.. ICC, IHC-P (5 µg/ml), IP, Peptide-ELISA (1:128000), WB (0.01 - 0.03 µg/ml) Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 80 kDa band observed in Mouse Brain lysates (calculated MW of 70.8 kDa according to NP_001005743.1). Recommended concentration: 0.03-0.1 ug/ml.
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8650
  • Target name
    NUMB
  • Target description
    NUMB endocytic adaptor protein
  • Target synonyms
    C14orf41, S171, c14_5527, protein numb homolog, h-Numb, numb homolog
  • Host
    Goat
  • Reactivity
    Bovine, Canine, Chicken, Equine, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Rabbit, Xenopus
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NUMB using anti-NUMB antibody (A01206-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87 MG whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat brain tissue tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUMB antigen affinity purified polyclonal antibody (Catalog # A01206-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUMB at approximately 72-75 kDa. The expected band size for NUMB is at 42 kDa.
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