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ProductsBack/IHC-plus(tm) NUP210 / gp210 Antibody

IHC-plus(tm) NUP210 / gp210 Antibody

LS-B12271
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNUP210
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) NUP210 / gp210 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (10 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23225
  • Target name
    NUP210
  • Target description
    nucleoporin 210
  • Target synonyms
    GP210, POM210, nuclear pore membrane glycoprotein 210, nuclear envelope pore membrane protein POM 210, nuclear pore protein gp210, nucleoporin 210kDa, nucleoporin Nup210, pore membrane protein of 210 kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GP210/NUP210 using anti-GP210/NUP210 antibody (A05308). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: rat thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GP210/NUP210 antigen affinity purified polyclonal antibody (Catalog # A05308) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GP210/NUP210 at approximately 250 kDa. The expected band size for GP210/NUP210 is at 205 kDa.
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