IHC-plus(tm) PCNA Antibody

LifeSpan BioSciences

IHC-plus(tm) PCNA Antibody

23

A BLAST analysis was used to suggest cross reactivity with PCNA protein from human, monkey, dog, mouse, rat, bovine, chicken, fish and Xenopus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not kno

Immunohistochemistry: LS-B402 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B402 was determined to be 2.5 ug/ml.. ELISA (1:2000 - 1:8000), IHC, IHC-P (2.5 µg/ml), WB (1:500 - 1:2000) Immunohistochemistry: LS-B402 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B402 was determined to be 2.5 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1.69 mg/ml

Unconjugated

...

PCNA

proliferating cell nuclear antigen

ATLD2; cyclin; DNA polymerase delta auxiliary protein; proliferating cell nuclear antigen

Rabbit

IgG

Bovine, Canine, Chicken, Fish, Human, Monkey, Mouse, Rat, Xenopus

12352203