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293T lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
293T lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
293T lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

PCNA Polyclonal Antibody

BS-0754R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPCNA
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    PCNA Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5111
  • Target name
    PCNA
  • Target description
    proliferating cell nuclear antigen
  • Target synonyms
    ATLD2; cyclin; DNA polymerase delta auxiliary protein; proliferating cell nuclear antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12004
  • Protein Name
    Proliferating cell nuclear antigen
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Multiboosting of Cancer Immunotherapy by a Core-Shell Delivery System. Shao F et al., 2020 Jan 6, Mol Pharm
    Read more
  • Down-regulation of Suv39h1 attenuates neointima formation after carotid artery injury in diabetic rats. Zhang J et al., 2020 Jan, J Cell Mol Med
    Read more
  • Astragalus Polysaccharide Improves Insulin Sensitivity via AMPK Activation in 3T3-L1 Adipocytes. Zhang R et al., 2018 Oct 21, Molecules
    Read more
  • Repression of Dok7 expression mediated by DNMT1 promotes glioma cells proliferation. Hua CD et al., 2018 Oct, Biomed Pharmacother
    Read more
  • Long non-coding RNA SPRY4-IT1 promotes the proliferation and invasion of U251 cells through upregulation of SKA2. He XJ et al., 2018 Mar, Oncol Lett
    Read more
  • The molecular mechanism of cell cycle arrest in the Bursa of Fabricius in chick exposed to Aflatoxin B 1. Hu P et al., 2018 Jan 29, Sci Rep
    Read more
  • The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum. Yin H et al., 2016 Jun 14, Oncotarget
    Read more
  • Effects of autologous bone marrow-derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats. Bi L et al., 2015 Sep, Exp Ther Med
    Read more
  • Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice. Zhang JY et al., 2015 Apr 14, World J Gastroenterol
    Read more
  • Urotensin II promotes atherosclerosis in cholesterol-fed rabbits. Li Y et al., 2014, PLoS One
    Read more

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Figure 1. Western blot analysis of PCNA using anti-PCNA antibody (A00125). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCNA antigen affinity purified polyclonal antibody (Catalog # A00125) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCNA at approximately 36 kDa. The expected band size for PCNA is at 29 kDa.
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