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293T lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
293T lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
293T lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

PCNA Polyclonal Antibody

BS-0754R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPCNA
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Overview

  • Supplier
    Bioss
  • Product Name
    PCNA Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5111
  • Target name
    PCNA
  • Target description
    proliferating cell nuclear antigen
  • Target synonyms
    ATLD2, proliferating cell nuclear antigen, DNA polymerase delta auxiliary protein, cyclin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12004
  • Protein Name
    Proliferating cell nuclear antigen
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Shao F, Zhang M, Xu L, et al. Multiboosting of Cancer Immunotherapy by a Core-Shell Delivery System. Mol Pharm. 2020,17(1):338-348. doi: 10.1021/acs.molpharmaceut.9b01113
    Read this paper
  • Zhang J, Yang J, Xu C, et al. Down-regulation of Suv39h1 attenuates neointima formation after carotid artery injury in diabetic rats. J Cell Mol Med. 2020,24(1):973-983. doi: 10.1111/jcmm.14809
    Read this paper
  • Zhang R, Qin X, Zhang T, et al. Astragalus Polysaccharide Improves Insulin Sensitivity via AMPK Activation in 3T3-L1 Adipocytes. Molecules. 2018,23(10). doi: 10.3390/molecules23102711
    Read this paper
  • Hua CD, Bian EB, Chen EF, et al. Repression of Dok7 expression mediated by DNMT1 promotes glioma cells proliferation. Biomed Pharmacother. 2018,106:678-685. doi: 10.1016/j.biopha.2018.06.156
    Read this paper
  • He XJ, Bian EB, Ma CC, et al. Long non-coding RNA SPRY4-IT1 promotes the proliferation and invasion of U251 cells through upregulation of SKA2. Oncol Lett. 2018,15(3):3977-3984. doi: 10.3892/ol.2018.7776
    Read this paper
  • Hu P, Zuo Z, Li H, et al. The molecular mechanism of cell cycle arrest in the Bursa of Fabricius in chick exposed to Aflatoxin B (1). Sci Rep. 2018,8(1):1770. doi: 10.1038/s41598-018-20164-z
    Read this paper
  • Yin H, Jiang M, Peng X, et al. The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum. Oncotarget. 2016,7(24):35592-35606. doi: 10.18632/oncotarget.9594
    Read this paper
  • Bi L, Wang G, Yang D, et al. Effects of autologous bone marrow-derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats. Exp Ther Med. 2015,10(3):851-856.
    Read this paper
  • Zhang JY, Song SD, Pang Q, et al. Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice. World J Gastroenterol. 2015,21(14):4195-209. doi: 10.3748/wjg.v21.i14.4195
    Read this paper
  • Li Y, Zhao S, Wang Y, et al. Urotensin II promotes atherosclerosis in cholesterol-fed rabbits. PLoS One. 2014,9(4):e95089. doi: 10.1371/journal.pone.0095089
    Read this paper

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Figure 1. Western blot analysis of PCNA using anti-PCNA antibody (A00125). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCNA antigen affinity purified polyclonal antibody (Catalog # A00125) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCNA at approximately 36 kDa. The expected band size for PCNA is at 29 kDa.
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