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IHC-plus(tm) PDIA2 Antibody (aa337-348)

LS-B4348
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPDIA2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PDIA2 Antibody (aa337-348)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:64000), WB (0.01 - 0.03 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64714
  • Target name
    PDIA2
  • Target description
    protein disulfide isomerase family A member 2
  • Target synonyms
    PDA2, PDI, PDIP, PDIR, protein disulfide-isomerase A2, Rho GDP dissociation inhibitor gamma, pancreas-specific protein disulfide isomerase, pancreatic protein disulfide isomerase, protein disulfide isomerase, pancreatic, protein disulfide isomerase-associated 2
  • Host
    Goat
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PDIP/PDIA2 using anti-PDIP/PDIA2 antibody (A03275-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDIP/PDIA2 antigen affinity purified polyclonal antibody (Catalog # A03275-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDIP/PDIA2 at approximately 65 kDa. The expected band size for PDIP/PDIA2 is at 68 kDa.
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