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ProductsBack/IHC-plus(tm) PKDCC / SGK493 Antibody

IHC-plus(tm) PKDCC / SGK493 Antibody

LS-B5068
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPKDCC
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PKDCC / SGK493 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    VLK antibody can be used for detection of VLK by Western blot at 0.5 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Western Blot: Predicted: 32, 54 kDa Observed: 31, 55 kDa. ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml) VLK antibody can be used for detection of VLK by Western blot at 0.5 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Western Blot: Predicted: 32, 54 kDa Observed: 31, 55 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID91461
  • Target name
    PKDCC
  • Target description
    protein kinase domain containing, cytoplasmic
  • Target synonyms
    RLSDF, SGK493, Vlk, extracellular tyrosine-protein kinase PKDCC, protein kinase domain containing, cytoplasmic homolog, protein kinase domain-containing protein, cytoplasmic, protein kinase-like protein SgK493, sugen kinase 493, vertebrate lonesome kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SGK493/PKDCC using anti-SGK493/PKDCC antibody (A11391-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGK493/PKDCC antigen affinity purified polyclonal antibody (Catalog # A11391-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGK493/PKDCC at approximately 54 kDa. The expected band size for SGK493/PKDCC is at 54 kDa.
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TargetPKDCC
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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGK493 antibody [N2C2], Internal (GTX119593) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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