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ProductsBack/SGK493 antibody [N2C2], Internal
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGK493 antibody [N2C2], Internal (GTX119593) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGK493 antibody [N2C2], Internal (GTX119593) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGK493 antibody [N2C2], Internal (GTX119593) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

SGK493 antibody [N2C2], Internal

GTX119593
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPKDCC
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Overview

  • Supplier
    GeneTex
  • Product Name
    SGK493 antibody [N2C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.61 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID91461
  • Target name
    PKDCC
  • Target description
    protein kinase domain containing, cytoplasmic
  • Target synonyms
    RLSDF, SGK493, Vlk, extracellular tyrosine-protein kinase PKDCC, protein kinase domain containing, cytoplasmic homolog, protein kinase domain-containing protein, cytoplasmic, protein kinase-like protein SgK493, sugen kinase 493, vertebrate lonesome kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ504Y2
  • Protein Name
    Extracellular tyrosine-protein kinase PKDCC
  • Scientific Description
    Protein kinase which is required for longitudinal bone growth through regulation of chondrocyte differentiation. Involved in protein transport from the Golgi apparatus to the plasma membrane.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SGK493/PKDCC using anti-SGK493/PKDCC antibody (A11391-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGK493/PKDCC antigen affinity purified polyclonal antibody (Catalog # A11391-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGK493/PKDCC at approximately 54 kDa. The expected band size for SGK493/PKDCC is at 54 kDa.
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