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IHC-plus(tm) PLA2G2A / SPLA2 Antibody

LS-B15952
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPLA2G2A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PLA2G2A / SPLA2 Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (5 µg/ml), Peptide-ELISA (1:8000), WB (0.1 - 0.3 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5320
  • Target name
    PLA2G2A
  • Target description
    phospholipase A2 group IIA
  • Target synonyms
    MOM1, PLA2, PLA2B, PLA2L, PLA2S, PLAS1, sPLA2, phospholipase A2, membrane associated, GIIC sPLA2, NPS-PLA2, group IIA phospholipase A2, non-pancreatic secretory phospholipase A2, phosphatidylcholine 2-acylhydrolase 2A, phospholipase A2, group IIA (platelets, synovial fluid)
  • Host
    Goat
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of PLA2G2A using anti-PLA2G2A antibody (M02259-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat small intestine tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse small intestine tissue lysates, Lane 8: mouse EL-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2G2A antigen affinity purified monoclonal antibody (Catalog # M02259-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLA2G2A at approximately 16 kDa. The expected band size for PLA2G2A is at 16 kDa.
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