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ProductsBack/IHC-plus(tm) POLR3F Antibody

IHC-plus(tm) POLR3F Antibody

LS-B4985
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPOLR3F
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) POLR3F Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    POLR3F antibody can be used for detection of POLR3F by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:. ELISA, IF, IHC, IHC-P (5 µg/ml), WB (0.5 - 1 µg/ml) POLR3F antibody can be used for detection of POLR3F by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10621
  • Target name
    POLR3F
  • Target description
    RNA polymerase III subunit F
  • Target synonyms
    C34, IMD101, RPC39, RPC6, DNA-directed RNA polymerase III subunit RPC6, RNA polymerase III C39 subunit, RNA polymerase III subunit C6, polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa, polymerase (RNA) III subunit F
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLR3F using anti-POLR3F antibody (A12720-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR3F antigen affinity purified polyclonal antibody (Catalog # A12720-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR3F at approximately 36 kDa. The expected band size for POLR3F is at 36 kDa.
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